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- SMAD1 Antibodies
Proteintech
View all (59) SMAD1 antibodies
Cite Phospho-SMAD1 (Ser214) Recombinant Rabbit Monoclonal Antibody (3K8)
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Phospho-SMAD1 (Ser214) Antibody (81433-1-RR) in WB
Non-treated and Calyculin A treated HepG2 cells were subjected to SDS PAGE followed by western blot with 81433-1-RR (Phospho-SMAD1 (Ser214) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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Product Details
81433-1-RR
Applications
Tested Dilution
Publications
Western Blot(WB)
1:2,000-1:16,800
-
ELISA(ELISA)
Assay-dependent
-
Product Specifications
Species Reactivity
Human,Mouse
Host/Isotype
Rabbit/ IgG
Expression System
Mammalian cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
3K8
Immunogen
Peptide
Conjugate
Unconjugated
Form
Liquid
Purification
Protein A
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
Target Information
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases:BSP-1; Dwarfin-A; Dwf-A; DWFC; hSMAD1; JV4-1; MAD hom*olog 1; MAD, mothers against decapentaplegic hom*olog 1; Mad-related protein 1; MADH5; mMad1; Mothers against decapentaplegic hom*olog 1; mothers against DPP hom*olog 1; Mothers-against-DPP-related 1; Mothers-against-DPP-related-1; OTTHUMP00000220456; SMAD; SMAD 1; SMAD family member 1; SMAD, mothers against DPP hom*olog 1; SMAD5; TGF-beta signaling protein 1; transforming growth factor-beta signaling protein 1; Transforming growth factor-beta-signaling protein 1
Gene Aliases:AI528653; BSP-1; BSP1; JV4-1; JV41; MADH1; MADR1; Mlp1; MusMLP; SMAD1
UniProt ID:(Human) Q15797, (Mouse) P70340
Entrez Gene ID:(Human) 4086, (Mouse) 17125
Molecular Function: 
DNA-binding transcription factor gene-specific transcriptional regulator
Function(s)
MAPK cascadeureteric bud developmentmesodermal cell fate commitmentosteoblast fate commitmenttranscription from RNA polymerase II promoterinflammatory responsesignal transductiontransforming growth factor beta receptor signaling pathwaySMAD protein complex assemblygamete generationnegative regulation of cell proliferationembryonic pattern specificationpositive regulation of gene expressionBMP signaling pathwaymidbrain developmenthindbrain developmentprimary miRNA processinghomeostatic processpositive regulation of osteoblast differentiationpositive regulation of transcription from RNA polymerase II promotercartilage developmentcardiac muscle cell proliferationbone developmentSMAD protein signal transductionpositive regulation of cartilage developmentpositive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulustranscription, DNA-templatedregulation of transcription, DNA-templatedregulation of transcription from RNA polymerase II promoterprotein phosphorylationresponse to drugpositive regulation of cell differentiationcellular response to organic cyclic compoundcellular response to BMP stimulus
Process(es)
RNA polymerase II core promoter proximal region sequence-specific DNA bindingRNA polymerase II core promoter sequence-specific DNA bindingtranscriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific bindingtranscription factor activity, sequence-specific DNA bindingreceptor signaling protein activityprotein bindingprotein kinase bindingtransforming growth factor beta receptor, pathway-specific cytoplasmic mediator activityidentical protein bindingprotein hom*odimerization activitymetal ion bindingprotein heterodimerization activityco-SMAD bindingI-SMAD bindingDNA binding
It has to be done as per old AB suggested Products section.
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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